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http://downloadeyebeamwithserialkey.com script to encode the file and download it from downloadeyebeamwithserialkey.com downloadeyebeamwithserialkey.com http://downloadeyebeamwithserialkey.com if you use the script, you can download the file with serial key only. [expression of c-fos, c-jun and c-myc genes in adeno-associated virus gene therapy of a lung cancer cell line a549]. to study the expression of c-fos, c-jun and c-myc genes in a549 cells transfected with recombinant adeno-associated virus (raav) which was carrying transcriptional inhibitors fos and jun to analyze the inhibitory mechanism of raav gene therapy in lung cancer cells and tumor growth. lung cancer cells were transfected with raav-5c-fos, raav-5c-jun or raav-5c-myc to construct the raav and the expression of fos, jun and myc proteins were detected by western blot. cell growth curve was drawn in each group. the mrna expression of c-fos, c-jun and c-myc in each group were determined by reverse transcriptase-polymerase chain reaction (rt-pcr). the effect of raav on the proliferation of tumor cells and the growth of subcutaneous tumors in nude mice were evaluated by mtt assay and subcutaneous inoculation of tumor cells in nude mice, respectively. the expression of fos, jun and myc proteins in lung cancer cells were suppressed by the raav transfection. the mtt assay showed that the transfection with raav had no obvious effects on the proliferation of the lung cancer cells. the growth rate of the subcutaneous tumors transfected with raav decreased with the increase of the viral titer and the expression of c-fos and c-jun genes. the expression of c-fos and c-jun genes in lung cancer cells transfected with raav-5c-fos, raav-5c-jun and raav-5c-myc were decreased by 87.03%, 87.67% and 100%, respectively. the inhibitory rate of tumor growth was 90.72%, 89.45% and 87.54% for raav-5c-fos, raav-5c-jun and raav-5c-myc, respectively. the expression of c-fos and c-jun genes in lung cancer cells can be suppressed by raav gene therapy, which is partly due to the inhibition of the transcription of the c-fos and c-jun genes and partly due to other inhibitory mechanisms. the subcutaneous tumor growth of nude mice was suppressed by raav transfection in a concentration-dependent manner. raav-5c-fos, raav-5c-jun and raav-5c-myc are able to suppress the expression of c-fos, c-jun and c-myc genes in lung cancer cells. the tumor growth of nude mice was suppressed.
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